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KMID : 1140120090140030256
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Cancer Prevention Research
2009 Volume.14 No. 3 p.256 ~ p.263
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Apoptosis Induced by Widdrol in Human Lung Carcinoma Cells
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Oh You-Na
Park Cheol
Kwon Hyun-Ju
Kim Byung-Woo
Choi Yung-Hyun
Yoon Hee-Jung
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Abstract
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Widdrol, a crystalline, natural substance, is an odorant derivative contained in various plants such as the Juniperus sp., including Juniperus chinensis. Though widdrol has been known to possess anti-fungal activity against Botrytis cinerea, anti-cancer properties of widdrol are not well understood. Therefore, we report the cytotoxic activity and the mechanism of cell death exhibited by the widdrol from Juniperus chinensis against human lung carcinoma A549 cells. Treatment A549 cells with various concentrations of widdrol resulted in growth inhibition and apoptosis induction in a dose-dependent manner as determined by cell viability, chromatin condensation, and DNA fragmentation analysis. The Western blot analysis revealed that apoptosis in A549 cells was associated with release of cytochrome c from mitochondria into the cytosol, activation of caspase-3, -8, -9 and proteolytic cleavage of poly (ADP-ribose) polymerase (PARP). These results suggest that widdrol exhibits antiproliferative effect on A549 cells via apoptosis, and can be a potential candidate in the field of anticancer drug discovery.
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KEYWORD
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Widdrol, A549, Apoptosis, Caspase, DNA fragmentation
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